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JoVE Journal
Immunology and Infection

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Merkel Cell Polyomavirus Infection and Detection

Merkel Cell Polyomavirus Infection and Detection

Article doi: 10.3791/58950
February 7th, 2019

Summary February 7th, 2019

Here, we present a protocol to infect primary human dermal fibroblast with MCPyV. The protocol includes isolation of dermal fibroblasts, preparation of MCPyV virions, virus infection, immunofluorescence staining, and fluorescence in situ hybridization. This protocol can be extended for characterizing MCPyV-host interactions and discovering other cell types infectable by MCPyV.


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