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Morphological and Functional Evaluation of Ribbon Synapses at Specific Frequency Regions of the Mouse Cochlea
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JoVE Journal Neuroscience
Morphological and Functional Evaluation of Ribbon Synapses at Specific Frequency Regions of the Mouse Cochlea

Morphological and Functional Evaluation of Ribbon Synapses at Specific Frequency Regions of the Mouse Cochlea

DOI:
10.3791/59189-v

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09:54 min

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May 10, 2019

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, , , , , , , , ,
1Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital,Capital Medical University, 2Department of Otology,Shengjing Hospital of China Medical University

Chapters

  • 00:04Title
  • 00:50Animal Selection
  • 02:12Cochlear Tissue Processing
  • 04:08Immunofluorescence Staining
  • 05:53Morphological Cochlear Ribbon Synapse Evaluation
  • 07:37Results: Representative Cochlear Frequency Localization and Cochlear Ribbon Analysis
  • 09:08Conclusion

Summary

Automatic Translation

Automatic Translation

This manuscript describes an experimental protocol for evaluating the morphological characteristics and functional status of ribbon synapses in normal mice. The present model is also suitable for noise-induced and age-related cochlear synaptopathy-restricted models. The correlative results of previous mouse studies are also discussed.

Tags

Keywords: CochleaFrequency RegionSynapseNeuropathyHearing LossTinnitusHyperacusisAuditory Brainstem ResponseABRCochlea DissectionParaformaldehydeEDTAMicroscopy

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