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JoVE Journal Bioengineering
3D analyse af multi-cellulære svar til Chemoattraktant gradienter
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3D analyse af multi-cellulære svar til Chemoattraktant gradienter

Article DOI: 10.3791/59226-v • 05:57 min • May 24th, 2019
May 24th, 2019 •
1, 2, 1, 2,3, 1
1Department of Biomedical Engineering and Yale Systems Biology Institute, Yale University, 2Department of Biomedical Engineering, Johns Hopkins University, 3Center for Cell Dynamics and Department of Cell Biology, Johns Hopkins University

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Vi beskriver en metode til at konstruere enheder til 3D-kultur og eksperimenter med celler og fler cellulære organoider. Denne enhed gør det muligt at analysere cellulære reaktioner på opløselige signaler i 3D-mikromiljøer med definerede chemoattraktant gradienter. Organoider er bedre end enkeltceller ved påvisning af svage støjende indgange.

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Bioteknik emission 147 gradient sensing organer-on-a-chip kemo taxaer stereolitografi ekstracellulær matrix udvikling organoider
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