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Analisi 3D delle risposte multi-cellulari ai gradienti chemoattractant

Article DOI: 10.3791/59226-v • 05:57 min • May 24th, 2019
May 24th, 2019 •
1, 2, 1, 2,3, 1
1Department of Biomedical Engineering and Yale Systems Biology Institute, Yale University, 2Department of Biomedical Engineering, Johns Hopkins University, 3Center for Cell Dynamics and Department of Cell Biology, Johns Hopkins University

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Descriviamo un metodo per costruire dispositivi per la cultura 3D e la sperimentazione con cellule e organoidi multicellulari. Questo dispositivo consente l'analisi delle risposte cellulari ai segnali solubili in microambienti 3D con gradienti chemioattrattante definiti. Gli organoidi sono meglio di cellule singole al rilevamento di ingressi rumorosi deboli.

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Bioingegneria problema 147 rilevamento gradiente organi-on-a-chip chemotaxis stereolitografia matrice extracellulare sviluppo organoidi
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