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JoVE Journal Engineering
Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery
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Synthesis, Functionalization, and Characterization of Fusogenic Porous Silicon Nanoparticles for Oligonucleotide Delivery

Article DOI: 10.3791/59440-v • 08:53 min • April 16th, 2019
April 16th, 2019 •
1, 1,2
1Materials Science and Engineering Program, University of California, San Diego, 2Department of Chemistry and Biochemistry, University of California, San Diego

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Summary
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We demonstrate the synthesis of fusogenic porous silicon nanoparticles for effective in vitro and in vivo oligonucleotide delivery. Porous silicon nanoparticles are loaded with siRNA to form the core, which is coated by fusogenic lipids through extrusion to form the shell. Targeting moiety functionalization and particle characterization are included.

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Synthesis Functionalization Characterization Fusogenic Porous Silicon Nanoparticles Oligonucleotide Delivery SiRNAs Targeting Peptide Therapeutic Formulation Gene Modulation Gram-positive Bacterial Infections Inflammation Macrophages Teflon Etch Cell Silicon Wafer Hydrofluoric Acid Solution Alternating Current Square Waveform Current Density Etching Ethanol Rinse Lift Off Porous Layer
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