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JoVE Journal
Neuroscience

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A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells
 

A Method for 3D Reconstruction and Virtual Reality Analysis of Glial and Neuronal Cells

Article doi: 10.3791/59444
September 28th, 2019 Usage Statistics

Summary September 28th, 2019

The described pipeline is designed for the segmentation of electron microscopy datasets larger than gigabytes, to extract whole-cell morphologies. Once the cells are reconstructed in 3D, customized software designed around individual needs can be used to perform a qualitative and quantitative analysis directly in 3D, also using virtual reality to overcome view occlusion.

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