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Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry
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Neuroscience
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JoVE Journal Neuroscience
Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

DOI:
10.3791/59683-v

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05:25 min

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July 01, 2019

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, , ,
1Department of Biomedical Sciences,University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine,University of Pennsylvania School of Veterinary Medicine

Chapters

  • 00:04Title
  • 00:45Mouse Eye Enucleation, Eye Cup Dissection, and Embedding
  • 03:08Sectioning Using a Cryostat
  • 03:51Results: Immunohistochemistry of Mouse Retinal Cryo-sections
  • 05:01Conclusion

Summary

Automatic Translation

Automatic Translation

This report describes comprehensive methods for preparing frozen mouse retina sections for immunohistochemistry (IHC). Methods described include dissection of the ocular posterior cup, paraformaldehyde fixation, embedding in Optimal Cutting Temperature (OCT) media and tissue orientation, sectioning and immunostaining.

Tags

Keywords: Mouse RetinaRetinal DissectionImmunohistochemistryCryo-sectionEye EnucleationEye FixationCornea RemovalLens ExtractionRetina IsolationCryo-protectionSnap Freezing

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