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Use of Micro X-ray Computed Tomography with Phosphotungstic Acid Preparation to Visualize Human Fibromuscular Tissue
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Bioengineering
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JoVE Journal Bioengineering
Use of Micro X-ray Computed Tomography with Phosphotungstic Acid Preparation to Visualize Human Fibromuscular Tissue

Use of Micro X-ray Computed Tomography with Phosphotungstic Acid Preparation to Visualize Human Fibromuscular Tissue

DOI:
10.3791/59752-v

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07:06 min

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September 05, 2019

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, , , ,
1Department of Anatomy,Yonsei University College of Medicine, 2Department of Anesthesiology and Pain Medicine, Anesthesia and Pain Research Institute,Yonsei University College of Medicine, 3Surgical Anatomy Education Centre at the Yonsei University College of Medicine

Chapters

  • 00:04Title
  • 00:41Sample Acquisition
  • 01:34Staining and Phosphotungstic Acid (PTA) Preparation and Micro-Computed Tomography (microCT) Scanning
  • 03:26Data Reconstruction and Optimization
  • 04:213D Reconstruction
  • 05:31Results: Representative 3D Orbicularis Retaining Ligament (ORL) Images
  • 06:23Conclusion

Summary

Automatic Translation

Automatic Translation

Micro X-ray computed tomography is effective in obtaining three-dimensional information from undamaged human specimens but has limited success in observing soft tissues. The use of phosphotungstic acid contrast agent can resolve this issue. We implemented this contrast agent to examine human delicate fibromuscular tissues (the orbicularis retaining ligament).

Tags

Keywords: MicroCTPhosphotungstic AcidOrbicularis Retaining LigamentSample PreparationMicroCT Scanning

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