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Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease
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Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Article DOI: 10.3791/62909-v • 12:15 min • February 8th, 2022
February 8th, 2022 •
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1Centre de recherche Cardio-Thoracique de Bordeaux, Univ. Bordeaux, 2IHU Liryc, Electrophysiology and Heart Modeling Institute, Fondation Bordeaux Univ., 3Electrophysiology and Ablation Unit, Bordeaux University Hospital (CHU)

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Summary
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Here, we present a protocol to obtain high resolution micro computed tomography images of healthy and pathological large mammalian whole hearts with collagen-selective contrast enhancement.

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Tissue Preparation Techniques Contrast-enhanced Micro Computed Tomography Imaging Large Mammalian Cardiac Models Chronic Disease Micro Structural Insight Whole Organ Scale High Resolution Imaging Translational Large Mammalian Models Selective Contrast Enhancement Structural Diseases Cardiac Microstructure Fibrosis Distribution Excitable Myocardium Organization Arrhythmia Cardioplegic Solution Sodium Heparin PBS/EDTA Solution EDTA Concentration PH Adjustment Sodium Hydroxide Hydrochloric Acid PBS Solution Room Temperature Storage 1% Ethanol PMA Contrast Agent Solution
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