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Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease
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Medicine
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JoVE Journal Medicine
Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

DOI:
10.3791/62909-v

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12:15 min

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February 08, 2022

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, , , , , , , , , , , , , , ,
1Centre de recherche Cardio-Thoracique de Bordeaux,Univ. Bordeaux, 2IHU Liryc, Electrophysiology and Heart Modeling Institute,Fondation Bordeaux Univ., 3Electrophysiology and Ablation Unit,Bordeaux University Hospital (CHU)

Chapters

  • 00:04Introduction
  • 00:54Solution Preparation
  • 02:05Tissue Preparation
  • 05:21Tissue Dehydration and Drying
  • 06:57MicroCT
  • 09:20Results: Contrast-Enhanced Air-Dried Tissue Preparations Enabled Microscale Imaging of Intact Large Mammalian Cardiac Models with Chronic Disease
  • 11:36Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to obtain high resolution micro computed tomography images of healthy and pathological large mammalian whole hearts with collagen-selective contrast enhancement.

Tags

Keywords: Micro Computed TomographyCardiac ModelsChronic DiseaseContrast EnhancementLarge MammalianMicrostructureFibrosisArrhythmiaCardioplegic SolutionPBS/EDTAPMA Contrast AgentCoronary OsteoPTFE Tubing

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