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X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease

X-ray Diffraction of Intact Murine Skeletal Muscle as a Tool for Studying the Structural Basis of Muscle Disease

DOI:
10.3791/59559-v

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08:26 min

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July 18, 2019

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,
1BioCAT, Dept. of Biological Sciences,Illinois Institute of Technology

Chapters

  • 00:04Title
  • 00:59Muscle Preparation
  • 03:15Muscle Mounting
  • 04:00Stimulation and Muscle Length Optimization
  • 05:00X-ray Diffraction
  • 06:07Extensor Digitorium Longus (EDL) X-ray Diffraction Patterns and Analysis
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

We present detailed protocols for performing small-angle X-ray diffraction experiments using intact mouse skeletal muscles. With the wide availability of transgenic mouse models for human diseases, this experimental platform can form a useful test bed for elucidating the structural basis of genetic muscle diseases

Tags

Keywords: X-ray DiffractionMurine Skeletal MuscleMuscle DiseaseStructural BasisPhysiological ConditionsGenetically Modified ModelsMyopathiesExtensor Digitorum LongusSoleus MuscleDissectionRinger’s SolutionGastrocnemius MuscleTibialis Anterior Muscle

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