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Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

Integrated Cell Manipulation Platform Coupled with the Single-probe for Mass Spectrometry Analysis of Drugs and Metabolites in Single Suspension Cells

DOI:
10.3791/59875-v

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07:55 min

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June 21, 2019

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, , , ,
1Department of Chemistry and Biochemistry,University of Oklahoma

Chapters

  • 00:04Title
  • 00:42Glass Cell-Selection Probe Fabrication and Integrated Cell Manipulation Platform Assembly
  • 02:32Couple the ICMP with a Single-Probe Setup and Suspended Cell Sample Preparation
  • 03:58Perform SCMS Measurements Using the ICMP/Single-probe Setup
  • 05:57Results: Integrated Cell Manipulation Platform Testing of Single Suspension Cells
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

An integrated cell manipulation platform is developed for use in conjunction with a single-probe mass spectrometry setup for the on-line analysis of individual suspension cells under ambient conditions.

Tags

Keywords: Single Cell AnalysisSingle-probe Mass SpectrometryIntegrated Cell Manipulation PlatformLiquid BiopsyCellular HeterogeneitySample PreparationPatient-derived CellsAmbient AnalysisGlass Probe FabricationMicroinjector Setup

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