Patient-derived Cells
Patient-derived Cells - Related Videos
06:48
In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)
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2025
This protocol describes in ovo xenografting of patient-derived B- and T-acute lymphoblastic leukemia (ALL) cells, which occurs 4 days following injection.
12:16
Patient Derived Cell Culture and Isolation of CD133+ Putative Cancer Stem Cells from Melanoma
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Cited by 22 •
2013
This article describes the preparation of freshly obtained melanoma tissue into primary cell cultures, and how to remove contaminations of erythrocytes and fibroblasts from the tumor cells. Finally, we describe how CD133+ putative melanoma stem cells are sorted from the CD133- bulk using Magnetic Activated Cell Sorting (MACS).
11:26
Isolation and Immortalization of Patient-derived Cell Lines from Muscle Biopsy for Disease Modeling
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Cited by 19 •
2015
This protocol describes techniques for live cell isolation and primary culture of myogenic and fibroblast cell lines from muscle or skin tissue. A technique for the immortalization of these cell lines is also described. Altogether, these protocols provide a reliable tool to generate and preserve patient-derived cells for downstream applications.
08:03
Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells
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Cited by 59 •
2015
This manuscript details a method used to generate prostate cancer patient derived xenografts (PDXs) from circulating tumor cells (CTCs). The generation of PDX models from CTCs provides an alternative experimental model to study prostate cancer; the most commonly diagnosed tumor and a frequent cause of death from cancer in men.
03:21
Zebrafish Patient-Derived Xenograft (zPDX): A Cancer Model
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2023
This video describes the technique to create a zebrafish patient derived xenograft model. In the sample protocol, we assess the efficiency of the drugs Gemcitabine and Navitoclax on the pancreatic tumor xenograft model.
FAQs
Their relevance comes from retaining genetic and cellular features associated with the person from whom they were collected. When cells from different individuals are studied under controlled laboratory conditions, researchers can examine how those differences relate to disease mechanisms or cellular responses. This makes the models useful for investigating biological variation rather than relying only on generalized cellular behavior.
Controlled culture conditions help researchers examine cellular behavior in a consistent laboratory environment, but they can also influence how the cells behave over time. Expansion, differentiation, or reprogramming may alter the model’s characteristics, so findings require careful interpretation. Monitoring and characterizing the cells helps determine whether they still represent the biology relevant to the research question.
The cells’ behavior may change as they are maintained or expanded outside the individual’s body. Such changes can affect how researchers interpret cellular responses and disease-related observations. For that reason, characterization is essential: it helps establish which genetic and cellular features remain relevant and supports more reliable conclusions about mechanisms, variation, or potential treatment effects.
A study generally begins by isolating cells from a tissue or clinical sample. Researchers then maintain them under controlled laboratory conditions and may expand, differentiate, or reprogram them according to the experimental goal. Before interpreting results, they characterize the resulting cells to assess their relevant features and confirm that the model is suitable for the intended investigation.
These models can reveal how cells respond under laboratory conditions and help investigators examine mechanisms associated with disease. Because the cells retain relevant features from an individual, comparisons can also highlight variation between people. The resulting information may support evaluation of potential treatments, provided that researchers account for changes introduced during laboratory maintenance or manipulation.
They are particularly useful when researchers need a laboratory model that reflects aspects of an individual’s biology. Applications include investigating disease mechanisms, studying cellular responses, comparing biological variation between individuals, and evaluating potential treatments. In each case, careful characterization strengthens interpretation because cultured cells may not behave exactly as they did in the original clinical or tissue context.