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Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization
JoVE Journal
Biology
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JoVE Journal Biology
Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization
DOI:

09:14 min

October 20, 2019

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Chapters

  • 00:04Title
  • 01:51Subcutaneous Abdominal Adipose Tissue Collection
  • 03:00Lipoaspirate Micro-Fragmentation
  • 05:30Micro-Fragmented Adipose Tissue (MAT) Digestion and Cell Isolation
  • 06:49Results: Representative MAT Characterization
  • 07:50Conclusion

Summary

Automatic Translation

Here, we present human adipose tissue enzyme-free micro-fragmentation using a closed system device. This new method allows the obtainment of sub-millimeter clusters of adipose tissue suitable for in vivo transplantation, in vitro culture, and further cell isolation and characterization.

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