Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

Bianca Vezzani; Mario Gomez-Salazar; Joan Casamitjana; Carlo Tremolada; Bruno P&#233;ault

doi:10.3791/60117

Journal

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Biology

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Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

JoVE Journal
Biology

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JoVE Journal Biology

Human Adipose Tissue Micro-fragmentation for Cell Phenotyping and Secretome Characterization

9,699 Views

•

09:14 min

•

October 20, 2019

DOI:

10.3791/60117-v

DOI:

10.3791/60117-v

•

09:14 min

October 20, 2019

•

9676 Views

Bianca Vezzani^1,2, Mario Gomez-Salazar¹, Joan Casamitjana¹, Carlo Tremolada³, Bruno Péault^1,4

¹MRC Center for Regenerative Medicine,University of Edinburgh ²Dept. of Morphology, Surgery and Experimental Medicine, Section of General Pathology,University of Ferrara ³Italian Image Institute ⁴Orthopaedic Hospital Research Center and Broad Stem Cell Research Center, David Geffen School of Medicine,University of California

Summary

Here, we present human adipose tissue enzyme-free micro-fragmentation using a closed system device. This new method allows the obtainment of sub-millimeter clusters of adipose tissue suitable for in vivo transplantation, in vitro culture, and further cell isolation and characterization.