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JoVE Journal Cancer Research
Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
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Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking

Article DOI: 10.3791/60426-v • 11:39 min • October 23rd, 2019
October 23rd, 2019 •
*1, *1, 1, 1
1Paul O'Gorman Leukaemia Research Centre, Institute of Cancer Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow
* These authors contributed equally

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Summary
Automatic Translation

This protocol enables the optimization and subsequent efficient generation of nuclear and cytoplasmic fractions from primary chronic lymphocytic leukemia cells. These samples are used to determine protein localization as well as changes in protein trafficking that take place between the nuclear and cytoplasmic compartments upon cell stimulation and drug treatment.

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Subcellular Fractionation Primary Chronic Lymphocytic Leukemia Cells Nuclear/cytoplasmic Protein Trafficking Transportation Of Macromolecules Mislocalization Tumor Suppressor Proteins CLL Pathogenesis Novel Therapies Nuclear And Cytoplasmic Fractions Enzyme Activity Assays Proteomic Analysis Western Blotting Shuttling Of Proteins B Cell Malignancies Location Of Proteins Protein Movement Detergent Gradient Highly Enriched Fractions Jodie Hay Michael Moles Jennifer Cassels Technician Peripheral Blood Samples EDTA Blood Collection Tubes
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