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Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum
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JoVE Journal Neuroscience
Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum

Purification of Prominin-1+ Stem Cells from Postnatal Mouse Cerebellum

DOI:
10.3791/60554-v

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07:04 min

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April 12, 2020

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1Davee Department of Neurology,Northwestern University Feinberg School of Medicine Chicago, 2Department of Cell and Molecular Biology,Northwestern University Feinberg School of Medicine Chicago

Chapters

  • 00:05Introduction
  • 00:53Cerebellum Dissection and Cell Suspension Preparation
  • 02:34Immunolabeling of Stem Cells
  • 03:44Magnetic Column Preparation and Cell Sorting
  • 04:22Passaging of Neurospheres and Differentiation
  • 05:27Results: Prominin-1 Stem Cells Isolated from Postnatal Cerebellum
  • 06:15Conclusion

Summary

Automatic Translation

Automatic Translation

Demonstrated here is an efficient and cost-effective method to purify, culture, and differentiate white matter stem cells from postnatal mouse cerebellum.

Tags

Keywords: Prominin-1Stem CellsCerebellumPostnatalFACSPapainDNAseCentrifugationCell Strainer

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