Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Separation of Bioactive Small Molecules, Peptides …
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method
An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract
Journal (Immunology and Infection)
An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract
An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics
Journal (Immunology and Infection)
An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics
 

Separation of Bioactive Small Molecules, Peptides from Natural Sources and Proteins from Microbes by Preparative Isoelectric Focusing (IEF) Method

Article DOI: 10.3791/61101-v • 09:57 min • June 14th, 2020
June 14th, 2020 •
1, 1, 1, 1
1Division of Biology, Kansas State University

Chapters

Summary
Automatic Translation

The objective is to fractionate and isolate bioactive small molecules, peptides from a complex plant extract, and proteins from pathogenic microbes by employing liquid-phase isoelectric focusing (IEF) method. Further, the separated molecules were identified and their bioactivity confirmed.

Tags

Separation Bioactive Small Molecules Peptides Natural Sources Proteins Preparative Isoelectric Focusing IEF Method Plant Extract Therapeutic Use Instrument Components Assembly Methods Sample Preparation Liquid Phase IEF Unit Anion Exchange Membranes Cation Exchange Membranes Rubber Gaskets Electrophoresis Inner Electrode Portion Outer Electrode Portion Ion Exchange Gaskets Electrolytes Plastic Screw Holder Sample Collection Board
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter