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High-Throughput Method for Measuring Alcohol Sedation Time of Individual Drosophila melanogaster
JoVE Journal
Behavior
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JoVE Journal Behavior
High-Throughput Method for Measuring Alcohol Sedation Time of Individual Drosophila melanogaster

High-Throughput Method for Measuring Alcohol Sedation Time of Individual Drosophila melanogaster

DOI:
10.3791/61108-v

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06:15 min

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April 20, 2020

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, , ,
1Department of Genetics and Biochemistry and Center for Human Genetics,Clemson University

Chapters

  • 00:04Introduction
  • 00:57Construction of the Testing Apparatus and Filming Chamber
  • 02:11Preparation of the Testing Apparatus and Flies
  • 03:32Testing the Flies
  • 04:49Results: Alcohol Sedation Times of DGRP Lines RAL_177 and RAL_555
  • 05:37Conclusion

Summary

Automatic Translation

Automatic Translation

Current methods to measure alcohol sensitivity in Drosophila are designed to test groups of flies. We present a simple, low-cost, high-throughput assay for assessing alcohol sedation sensitivity in large numbers of single flies. The method does not require specialized tools and can be performed in any laboratory using common materials.

Tags

Keywords: Alcohol SensitivityDrosophila MelanogasterHigh-throughputSingle-fly AssayAlcohol Sedation TimeGenetic UnderpinningsVolatilesInsect Screen MeshFilming ChamberControlled Environment

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