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A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila
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Behavior
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JoVE Journal Behavior
A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila

A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila

DOI:
10.3791/62771-v

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08:55 min

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June 14, 2021

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, , , ,
1Department of Genetics and Biochemistry and Center for Human Genetics,Clemson University

Chapters

  • 00:04Introduction
  • 00:45Starvation Plate Preparation, Fly Sorting, and Starvation
  • 02:26Liquid Food and Feeder Plate Preparation
  • 04:33Exposure
  • 07:09Results: Quantification of Consumption in Drosophila
  • 08:16Conclusion

Summary

Automatic Translation

Automatic Translation

The microplate feeder assay offers an economical, high throughput method for quantifying liquid food consumption in Drosophila. A 3D-printed device connects a 96-well microplate in which flies are housed to a 1536-well microplate from which flies consume a feeding solution with a tracer dye. The solution volume decline is measured spectrophotometrically.

Tags

Keywords: Microplate Feeder AssayDrosophilaFeeding BehaviorHigh-throughputConsumptionWater-soluble NutrientsDrugsPharmaceuticalsToxinsInsect SpeciesAgaroseBarrier StripsCouplersStarvation PlateCO2 AnesthesiaLiquid FoodSucroseYeast ExtractDyeSyringeFilterFeeder Plate

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