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Journal / Behavior / A High Throughput Microplate Feeder Assay for…
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Behavior

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JoVE Journal Behavior
A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila
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A High Throughput Microplate Feeder Assay for Quantification of Consumption in Drosophila

Article DOI: 10.3791/62771-v • 08:55 min • June 14th, 2021
June 14th, 2021 •
*1, *1, 1, 1, 1
1Department of Genetics and Biochemistry and Center for Human Genetics, Clemson University
* These authors contributed equally

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Summary
Automatic Translation

The microplate feeder assay offers an economical, high throughput method for quantifying liquid food consumption in Drosophila. A 3D-printed device connects a 96-well microplate in which flies are housed to a 1536-well microplate from which flies consume a feeding solution with a tracer dye. The solution volume decline is measured spectrophotometrically.

Tags

High-throughput Microplate Feeder Assay Quantification Consumption Drosophila Absorbance Plate Reader Data Extraction Nutrient Quantification Insect Species Agarose Multi-channel Pipette Barrier Strips Couplers Starvation Plate CO2 Anesthesia Fly Sorting
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