High-Throughput Small Molecule Drug Screening For Age-Related Sleep Disorders Using <em>Drosophila melanogaster</em>

Zheng Zhang; Yuedong Wang; Jiaying Zhao; Shifeng Han; Zi Chao Zhang; Yao Tian

doi:10.3791/65787

Journal

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Behavior

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High-Throughput Small Molecule Drug Screening For Age-Related Sleep Disorders Using Drosophila melanogaster

High-Throughput Small Molecule Drug Screening For Age-Related Sleep Disorders Using <em>Drosophila melanogaster</em>

JoVE Journal
Behavior

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JoVE Journal Behavior

High-Throughput Small Molecule Drug Screening For Age-Related Sleep Disorders Using Drosophila melanogaster

High-Throughput Small Molecule Drug Screening For Age-Related Sleep Disorders Using Drosophila melanogaster

2,215 Views

•

05:59 min

•

October 20, 2023

DOI:

10.3791/65787-v

DOI:

10.3791/65787-v

•

05:59 min

October 20, 2023

•

9 Views

Zheng Zhang¹, Yuedong Wang¹, Jiaying Zhao¹, Shifeng Han¹, Zi Chao Zhang¹, Yao Tian¹

¹School of Life Science and Technology, Key Laboratory of Developmental Genes and Human Disease,Southeast University

Transcript

Drug screening techniques include high-throughput screening, which uses automation for drug discovery, structure-based designs, and find receptor binding molecules based on the structure, and a fragment-based design, which uses nuclear magnetic resonance and mass spectrometry to optimize molecular fragments and identify the compounds. The DAM system measures fly sleep by detecting infrared beam bricks. However, this method can overestimate sleep if the flies remain static at one end of the tube, or if the fly is not highly mobile.

Furthermore, the order of any tested drug may impact the fly fruit intake, potentially skewing experimental results. Therefore, our experiment will incorporate various stretches to mitigate these factors and enhance accuracy. Compared with video analysis based methods, our method offers high-throughput capabilities, reduce the cost, and simplify operation.

So process of experimental data is also relatively straightforward, where the resulting graph visually represents the drug’s effects.

Summary

Presented is a protocol for high-throughput drug screening to improve sleep by monitoring the sleep behavior of fruit flies in an elderly Drosophila model.