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Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons
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Neuroscience
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JoVE Journal Neuroscience
Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

Single-Cell Electroporation across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neurons

DOI:
10.3791/61662-v

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06:21 min

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October 06, 2020

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, ,
1Brudnick Neuropsychiatric Research Institute, Department of Neurobiology,University of Massachusetts Medical School, 2Interdisciplinary Graduate Program,University of Massachusetts Medical School, 3UMMS MD/PhD Program,University of Massachusetts Medical School

Chapters

  • 00:04Introduction
  • 00:46Electroporation Preparation
  • 03:03Electroporation
  • 04:58Results: Representative Mouse Hippocampal Excitatory and Class-Specific Inhibitory Neuron Electroporation Efficiency
  • 05:45Conclusion

Summary

Automatic Translation

Automatic Translation

Presented here is a protocol for single-cell electroporation that can deliver genes in both excitatory and inhibitory neurons across a range of in vitro hippocampal slice culture ages. Our approach provides precise and efficient expression of genes in individual cells, which can be used to examine cell-autonomous and intercellular functions.

Tags

Keywords: Single-cell ElectroporationOrganotypic Slice CultureMouse HippocampusExcitatory NeuronsInhibitory NeuronsGene TransfectionNeurobiologyElectroporation ProtocolHigh Transfection EfficiencyCell-autonomous MechanismsTranssynaptic Protein InteractionsSlice Culture PreparationElectroporator SetupPipette PreparationSlice Culture IsolationTarget Cell Electroporation

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