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Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
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Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability

Article DOI: 10.3791/61733-v • 06:42 min • September 1st, 2020
September 1st, 2020 •
*1, *1, *1, 1,2
1Department of Biophysics, University of Texas Southwestern Medical Center, 2Center for Alzheimer's and Neurodegenerative Disease and Center for Systems Biology, University of Texas Southwestern Medical Center
* These authors contributed equally

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Summary
Automatic Translation

Presented here is a protocol for cryogenic transfer of frozen samples into the dynamic nuclear polarization (DNP) magic angle spinning (MAS) nuclear magnetic resonance (NMR) probe. The protocol includes directions for rotor storage prior to the experiment and directions for viability measurements before and after the experiment.

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Cryogenic Sample Loading Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer Cellular Viability DNP Assisted MAS NMR Spectroscopy Sample Handling Cryogenic Transfer Cold Rotor Moisture And Ice Build Up Cryogenic Vial Sapphire Rotor Controlled Rate Cooling Container Liquid Nitrogen Vacuum Flask
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