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Journal / Biology / Electron Cryotomography of Bacterial Cells
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Biology

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Electron Cryotomography of Bacterial Cells
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Electron Cryotomography of Bacterial Cells

Article DOI: 10.3791/1943 • 14:23 min • May 6th, 2010
May 6th, 2010 •
1, 1,2, 1, 1,2, 1,2, 2, 1, 1,2, 1,2, 1, 1,2, 1, 1, 1,2
1Division of Biology, California Institute of Technology - Caltech, 2Howard Hughes Medical Institute, California Institute of Technology - Caltech

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Summary
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We illustrate here how to use electron cryotomography (ECT) to study the ultrastructure of bacterial cells in near-native states, to "macromolecular" (~4 nm) resolution.

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Electron Cryotomography Bacterial Cells Cell Shapes Cell Polarity Genome Segregation Cell Division Imaging Technologies Fluorescence Light Microscopy X-ray Crystallography NMR Spectroscopy Ultrastructure Of Cells Three Dimensions (3D) Macromolecular Resolution Cryo Transmission Electron Microscope (cryoTEM) Low Temperature Imaging Vitreous State Cryogens Ethane Ethane/propane Mixtures High-pressure Freezing Cryo-sectioning Two-dimensional Projection Images Tomogram
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