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A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo
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JoVE Journal Developmental Biology
A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

A Protocol for Immunohistochemistry and RNA In-situ Distribution within Early Drosophila Embryo

DOI:
10.3791/61776-v

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14:34 min

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May 06, 2022

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, , , , , , , , , , , , , , ,
1National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China/CGDB,Shaanxi Normal University College of Life Sciences, 2Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Shaanxi Key Laboratory of Brain disorders, Institute of Basic & Translational Medicine,Xi’an Medical University, 3Ohio State University College of Medicine, 4Hematology-Oncology Division, Department of Medicine, Beth Israel Deaconess Medical Center,Harvard Medical School, 5University of Pennsylvania ULAR, 6University of Maryland School of Medicine, 7Columbia University Medical Center

Chapters

  • 00:05Introduction
  • 00:44Coating Slides
  • 01:41Embedding of Drosophila Embryos
  • 04:25Hematoxylin-Eosin Staining
  • 05:25Periodic Acid-Silver Methenamine Staining
  • 06:28Immunohistochemistry
  • 08:15In Situ Hybridization
  • 12:17Results
  • 13:32Discussion

Summary

Automatic Translation

Automatic Translation

Here, we describe a protocol for detection and localization of Drosophila embryo protein and RNA from collection to pre-embedding and embedding, immunostaining, and mRNA in situ hybridization.

Tags

Keywords: DrosophilaEmbryoImmunohistochemistryRNA In-situHistochemistryProtein LocalizationDevelopmental BiologyChorionGelatin CoatingBleach DechorionationN-heptaneMethanol Precipitation

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