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JoVE Journal
Immunology and Infection

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Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions
 

Label-Free Quantitative Proteomics Workflow for Discovery-Driven Host-Pathogen Interactions

Article DOI: 10.3791/61881-v
October 20th, 2020

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Summary October 20th, 2020

Here, we present a protocol to profile the interplay between host and pathogen during infection by mass spectrometry-based proteomics. This protocol uses label-free quantification to measure changes in protein abundance of both host (e.g., macrophages) and pathogen (e.g., Cryptococcus neoformans) in a single experiment.

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