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JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics
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Biochemistry
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JoVE Journal Biochemistry
JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

DOI:
10.3791/62796-v

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07:28 min

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October 19, 2021

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, , , , , , , ,
1Departments of Structural Biology and Developmental Neurobiology,St. Jude Children’s Research Hospital, 2Center for Proteomics and Metabolomics,St. Jude Children’s Research Hospital, 3Department of Computational Biology,St. Jude Children’s Research Hospital

Chapters

  • 00:04Introduction
  • 01:03Setup of JUMPn Software
  • 01:31Demo Run Using an Example Dataset
  • 06:04Results: Workflow of JUMPn Software and the Protein-Protein Interaction Database
  • 07:00Conclusion

Summary

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We present a systems biology tool JUMPn to perform and visualize network analysis for quantitative proteomics data, with a detailed protocol including data pre-processing, co-expression clustering, pathway enrichment, and protein-protein interaction network analysis.

Tags

Keywords: Protein Co-expressionClusteringNetwork AnalysisProteomicsJUMPn SoftwareCo-expression ClusteringPathway EnrichmentPPI Module DetectionUser-friendly InterfaceInteractive VisualizationPhosphoproteomicsInteractome DataWGCNA AlgorithmCo-expression PatternsTrends PlotPathway Ontology Enrichment Heatmap

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