This study aims to develop a novel human organotypic retinal culture (HORC) model that prevents compromising retinal integrity during explant handling. This is achieved by culturing the retina with the overlying vitreous and the underlying retinal pigment epithelium-choroid (RPE-choroid) and sclera.
Kuo, C. Y. J., Louie, H. H., Rupenthal, I. D., Mugisho, O. O. Characterization of a Novel Human Organotypic Retinal Culture Technique. J. Vis. Exp. (172), e62046, doi:10.3791/62046 (2021).