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In vivo-like Organotypic Murine Retinal Wholemount Culture

In vivo-like Organotypic Murine Retinal Wholemount Culture

Article DOI: 10.3791/1634
January 11th, 2010


Summary January 11th, 2010

This video article demonstrates the establishment of organotypic retinal wholemount cultures and a cytospin procedure for analysis of exogenously induced effects. Organotypic retinal wholemount cultures mimic the in vivo situation and significantly facilitate the accessibility of murine retinas for experimental manipulations while circumventing the disadvantages of classical murine animal models.

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