Journal
/
Immunology and Infection
/
Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals

Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals

DOI:
10.3791/62195-v

•

08:31 min

•

September 20, 2021

•

, ,
1Department of Ophthalmology and Visual Sciences,University of Illinois at Chicago, 2Department of Microbiology and Immunology,University of Illinois at Chicago

Chapters

  • 00:05Introduction
  • 01:14Whole Eye Porcine Cornea Isolation
  • 02:55Visualization
  • 03:32Vero Cell Preparation
  • 04:19Virus Collection
  • 05:21Virus Infection Quantification
  • 06:52Results: Representative Viral Spread Infection Evaluation
  • 07:38Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the use of a porcine cornea to test the antiviral efficacy of experimental drugs.

Tags

Herpes Simplex Virus-1 AntiviralsAnimal ModelsIn Vitro TestingCornea IsolationCornea MaintenanceVirus Infection17 GFP Virus

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62195?article_type=v

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy
check_url/62195?article_type=v

A Primary Neuron Culture System for the Study of Herpes Simplex Virus Latency and Reactivation
check_url/62195?article_type=v

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
check_url/62195?article_type=v

Assays for the Identification of Novel Antivirals against Bluetongue Virus
check_url/62195?article_type=v

Ex Vivo Infection of Murine Epidermis with Herpes Simplex Virus Type 1
check_url/62195?article_type=v

Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
check_url/62195?article_type=v

A Method to Test the Efficacy of Handwashing for the Removal of Emerging Infectious Pathogens
check_url/62195?article_type=v

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
check_url/62195?article_type=v

Ex Vivo Infection of Human Lymphoid Tissue and Female Genital Mucosa with Human Immunodeficiency Virus 1 and Histoculture
check_url/62195?article_type=v

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Read Article