Porcine Corneal Explant
Porcine Corneal Explant - Related Videos
04:11
Laser-assisted Selective Ablation of Porcine Corneal Endothelial Cells: A Technique to Induce Focused Porcine Corneal Endothelial Injury for Ex Vivo Studies
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2025
This video demonstrates a non-invasive laser-assisted procedure to selectively damage the corneal endothelial cells in the porcine eye. The developed ex vivo model of endothelial cell damage can be used to study diseases of the corneal endothelium.
08:31
Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals
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Cited by 5 •
2021
We describe the use of a porcine cornea to test the antiviral efficacy of experimental drugs.
05:00
Porcine Ex vivo Cornea Model of Bacterial Keratitis: A Technique to Establish Bacterial Infection in Corneal Epithelial Cells
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2025
This video describes a protocol to set up an ex vivo model for bacterial keratitis using a corneoscleral button dissected from the porcine eye. Inoculated bacteria traverse the corneal epithelial incisions to invade the underlying stroma and establish an infection within the corneal tissues that mimic the in vivo infection.
08:36
A Porcine Corneal Endothelial Organ Culture Model Using Split Corneal Buttons
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Cited by 4 •
2019
Here, a step-by-step protocol for the preparation and cultivation of porcine split corneal buttons is presented. As this organo-typically cultivated organ culture model shows cell death rates within 15 days, comparable to human donor corneas, it represents the first model allowing long-term cultivation of non-human corneas without adding toxic dextran.
06:25
Explant Culture for Developmental Studies
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2023
Explant culture is a technique in which living cells or tissues are removed from an embryo for continued development outside of the organism. This ex vivo approach allows researchers to manipulate and observe developing tissues in ways that are not possible in vivo. Once established, explant culture is frequently used to understand the role of genes and signaling molecules in organogenesis. This video will first introduce the basic principles of explant culture and demonstrate a protocol to...
FAQs
The native layered structure helps retain relationships between the epithelial barrier, underlying tissue, and local cellular environment. This preservation allows researchers to examine how microbes or inflammatory stimuli affect the cornea as an organized tissue rather than as isolated components. Findings can therefore connect epithelial disruption, immune activation, and tissue damage within the same experimental model.
Controlled exposure allows investigators to introduce microbes or inflammatory stimuli under defined experimental conditions and then assess the resulting tissue response. This approach supports focused analysis of pathogen–host interactions, epithelial barrier function, immune activation, and damage. It also makes the explant useful for comparing how different challenges or interventions influence corneal tissue behavior.
A porcine corneal explant can support investigation of several connected processes, including weakening of the epithelial barrier, interactions between pathogens and host tissue, activation of local immune responses, and structural damage. Examining these outcomes together helps researchers relate an infectious or inflammatory challenge to the progression of tissue injury and to the effectiveness of protective treatments.
Researchers maintain a section of porcine corneal tissue outside the animal, expose it to a selected microbe or inflammatory stimulus, and evaluate the tissue response. Assessment can focus on epithelial barrier function, pathogen–host interactions, immune activation, or tissue damage. The workflow provides a controlled platform for testing how the cornea responds to defined experimental challenges.
The model can be used to investigate antimicrobial strategies and interventions intended to protect or restore infected corneal tissue. After exposing the explant to a relevant microbial or inflammatory challenge, researchers can assess whether a treatment is associated with reduced tissue damage, improved barrier-related outcomes, or altered immune activation. These observations support preclinical evaluation of candidate approaches.
Porcine corneal explants connect infection biology with tissue-level immunity by preserving the local environment in which microbial exposure and inflammatory signaling occur. They allow researchers to examine immune activation alongside pathogen–host interactions and corneal damage, while reducing reliance on whole-animal experiments. This makes the approach relevant for studying disease mechanisms, treatment responses, and vaccine-related responses.