Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Immunology and Infection
Author Produced

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
 

Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines

Article DOI: 10.3791/62328-v
November 13th, 2021

Chapters

Summary November 13th, 2021

This protocol uses fluorescent reporters and cell sorting to simplify knock-in experiments in macrophage and T cell lines. Two plasmids are used for these simplified knock-in experiments, namely a CRISPR/Cas9- and DsRed2-expressing plasmid and a homologous recombination donor plasmid expressing EBFP2, which is permanently integrated at the Rosa26 locus in immune cells.

Transcript

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter