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Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes
JoVE Journal
Biology
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JoVE Journal Biology
Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes

Using the Fluorescent Dye, Rhodamine B, to Study Mating Competitiveness in Male Aedes aegypti Mosquitoes

DOI:
10.3791/62432-v

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07:10 min

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May 07, 2021

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, , ,
1Environmental Health Institute,National Environmental Agency

Chapters

  • 00:04Introduction
  • 00:41Preparation of Male and Female Mosquitoes
  • 01:11Preparation of 0.2% RhB Sucrose Solution and Feeding of Male Mosquitoes
  • 01:52Checking for RhB Fluorescence in Male Mosquitoes
  • 02:46Mating Competitiveness Assay
  • 05:36Results: Assessment of Mating Competitiveness in Male Mosquitoes
  • 06:27Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol for studying mating competitiveness of male Aedes aegypti using fluorescent dye as a marker. Female mosquitoes are exposed to both marked and unmarked males for copulation. Post mating, their spermathecae are examined under a fluorescence microscope to determine their mating partner.

Tags

Keywords: Rhodamine BAedes AegyptiMating CompetitivenessMosquito Population SuppressionQuality ControlStrain EvaluationMale MosquitoesFemale MosquitoesSucrose SolutionFluorescence MicroscopyMating Assay

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