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JoVE Journal
Immunology and Infection

This content is Open Access.

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes

Article DOI: 10.3791/4215-v 09:00 min September 17th, 2012
September 17th, 2012



Among the three mosquito genera, namely Anopheles, Aedes, and Culex, physical genome mapping techniques were established only for Anopheles, whose members possess readable polytene chromosomes. For the genera of Aedes and Culex, however, cytogenetic mapping remains challenging because of the poor quality of polytene chromosomes. Here we present a universal protocol for obtaining high-quality preparations of mitotic chromosomes and an optimized FISH protocol for all three genera of mosquitoes.


Fluorescent In Situ Hybridization FISH Mitotic Chromosomes Mosquitoes DNA Probes RNA Probes Physical Maps Genome Assemblies Polytene Chromosomes Chromosome-based Mapping Technique Anopheles Aedes Culex Transposable Elements Genomic Supercontigs Ae. Aegypti Cx. Quinquefasciatus Mitotic Chromosome Preparation Brain Ganglia Cell Lines
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