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JoVE Journal
Immunology and Infection

This content is Open Access.

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes
 

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes

Article doi: 10.3791/4215
September 17th, 2012

Summary September 17th, 2012

Among the three mosquito genera, namely Anopheles, Aedes, and Culex, physical genome mapping techniques were established only for Anopheles, whose members possess readable polytene chromosomes. For the genera of Aedes and Culex, however, cytogenetic mapping remains challenging because of the poor quality of polytene chromosomes. Here we present a universal protocol for obtaining high-quality preparations of mitotic chromosomes and an optimized FISH protocol for all three genera of mosquitoes.

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