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Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes
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Chapters
- 00:05Title
- 01:06Chromosome Preparation
- 04:17Fluorescent in situ Hybridization (FISH)
- 08:05FISH Results
- 08:40Conclusion
Among the three mosquito genera, namely Anopheles, Aedes, and Culex, physical genome mapping techniques were established only for Anopheles, whose members possess readable polytene chromosomes. For the genera of Aedes and Culex, however, cytogenetic mapping remains challenging because of the poor quality of polytene chromosomes. Here we present a universal protocol for obtaining high-quality preparations of mitotic chromosomes and an optimized FISH protocol for all three genera of mosquitoes.
Tags
Fluorescent In Situ HybridizationFISHMitotic ChromosomesMosquitoesDNA ProbesRNA ProbesPhysical MapsGenome AssembliesPolytene ChromosomesChromosome-based Mapping TechniqueAnophelesAedesCulexTransposable ElementsGenomic SupercontigsAe. AegyptiCx. QuinquefasciatusMitotic Chromosome PreparationBrain GangliaCell Lines
