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A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

DOI:
10.3791/62452-v

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07:39 min

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April 13, 2021

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, , , , ,
1Department of Developmental Biology,University of Pittsburgh, School of Medicine, 2Department of Molecular Medicine and Pathology, School of Medical Sciences,University of Auckland, 3Center for Critical Care Nephrology,University of Pittsburgh, School of Medicine

Chapters

  • 00:04Introduction
  • 00:59Setting up the Kidney Organoid Assay and Feeding by Half-Medium Change
  • 02:49Transfer of Embryoid Bodies to Stage II Medium
  • 04:49Transfer to Spinner Flask and Feeding
  • 05:37Setting up a 6-Well Magnetic Stir Plate
  • 06:14Results: Immunofluorescence of the Kidney Organoids
  • 06:51Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol to generate kidney organoids from human pluripotent stem cells (hPSCs). This protocol generates kidney organoids within two weeks. The resulting kidney organoids can be cultured in large-scale spinner flasks or multi-well magnetic stir plates for parallel drug-testing approaches.

Tags

Keywords: Kidney OrganoidsHuman Pluripotent Stem CellsHPSCsCell CultureRenal DevelopmentRenal DiseaseSimplified MethodCell DifferentiationEmbryoid BodiesCell Culture Techniques

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