A Simplified Method for Generating Kidney Organoids from Human Pluripotent Stem Cells

This method can be performed in any laboratory equipped for cell culture experiments. Using this method, we can further our understanding of renal development and disease pathways. This is a relatively simple and inexpensive method compared to others for generating kidney organoids.

The biggest challenge of this technique is maintaining high-quality human pluripotent stem cell culture. It is critical that the cells are maintained at less than 80%confluency and split regularly. Researchers new to human pluripotent stem cell culture should familiarize themselves with the stem cell lines before proceeding to differentiation.