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Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos
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Developmental Biology
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JoVE Journal Developmental Biology
Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos

Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos

DOI:
10.3791/65090-v

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07:35 min

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February 17, 2023

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, , , ,
1Department of Internal Medicine – Nephrology,Leiden University Medical Center, 2Einthoven Laboratory of Vascular and Regenerative Medicine,Leiden University Medical Center, 3IBPS, CNRS UMR7622, Developmental Biology Laboratory,Sorbonne Université, 4The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW),Leiden University Medical Center

Chapters

  • 00:04Introduction
  • 00:30Preparing Chicken Embryos for Transplantation
  • 02:14Intracelomic Transplantation on Day 4 of Incubation
  • 03:18Transplantation
  • 04:09Injection
  • 04:59Collecting Transplanted Organoids on Day 12 of Incubation
  • 06:12Results: Analysing the Transplanted Kidney Organoids
  • 07:07Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a detailed protocol for the transplantation of kidney organoids in the celomic cavity of chicken embryos. This method induces vascularization and enhanced maturation of the organoids within 8 days and can be used to study these processes in an efficient manner.

Tags

Keywords: Kidney OrganoidsVascularizationIntracoelomic TransplantationChicken EmbryosIn Vitro MaturationHIPSC-derivedEgg IncubationEmbryo VisualizationCoelom Access

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