Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Nano-Differential-Scanning-Fluorimetrie für das…
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Nano-Differential-Scanning-Fluorimetrie für das Screening in der fragmentbasierten Lead-Discovery
5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells (Video) | JoVE
Journal (Biochemistry)
5-Bromo-2'-Deoxyuridine (BrdU) Labeling of Mitochondrial DNA in HeLa Cells (Video) | JoVE
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
Journal (Biochemistry)
Covalent Binding of Antibodies to Cellulose Paper Discs and Their Applications in Naked-eye Colorimetric Immunoassays
 
Click here for the English version

Nano-Differential-Scanning-Fluorimetrie für das Screening in der fragmentbasierten Lead-Discovery

Article DOI: 10.3791/62469-v • 06:26 min • May 16th, 2021
May 16th, 2021 •
1, 1, 1, 2, 2, 2, 2, 1, 1
1Oncode Institute and Division of Biochemistry, the Netherlands Cancer Institute, 2Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Die Überwachung von Änderungen der Schmelztemperatur eines Zielproteins(z. B.Thermal Shift Assay, TSA) ist eine effiziente Methode zum Screening von Fragmentbibliotheken von einigen hundert Verbindungen. Wir präsentieren ein TSA-Protokoll, das eine robotergestützte Nano-Differential Scanning Fluorimetry (Nano-DSF) zur Überwachung der intrinsischen Tryptophanfluoreszenz und der Lichtrückstreuung für das Fragmentscreening implementiert.

Tags

Biochemie Ausgabe 171 Thermal Shift Assay Fragmentscreening Wirkstoffdesign SARS-CoV-2 Nsp5 Hec1 Mps1
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter