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Journal
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Cancer Research
/
使用患者源性肿瘤类器官的高通量
体外
检测
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
High-Throughput
In Vitro
Assay using Patient-Derived Tumor Organoids
Please note that all translations are automatically generated.
Click here for the English version.
使用患者源性肿瘤类器官的高通量
体外
检测
DOI:
10.3791/62668-v
•
06:25 min
•
June 14, 2021
•
Arisa Higa
,
Nobuhiko Takahashi
3
,
Gen Hiyama
,
Hirosumi Tamura
,
Hirotaka Hoshi
,
Kenju Shimomura
,
Shinya Watanabe
,
Motoki Takagi
1
FUJIFILM Wako Bio Solutions Corporation
,
2
Medical-Industrial Translational Research Center
,
Fukushima Medical University
,
3
Department of Bioregulation and Pharmacological Medicine
,
Fukushima Medical University
Chapters
00:04
Introduction
00:59
Patient-Derived Tumor Organoid Culture
02:25
High Throughput Assay System for Growth Inhibition
03:32
High Throughput Assay System with Cell Picking and Imaging for Growth Inhibition
04:30
Results: Estimation of Anticancer Effects on the Patient-Derived Tumor Organoids
05:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
开发了一种高精度
的体外
高通量测定系统,以使用患者来源的肿瘤类器官(PDO)评估抗癌药物,类似于癌症组织,但不适用于具有96孔和384孔板
的体外
高通量测定系统。
Tags
Patient-derived Tumor Organoids
High-throughput In Vitro Assay
Cell Analysis
2D Culture
3D Culture
Molecular Targeted Drugs
Cell Suspension
384-well Microplate
Liquid Handler
Cell Fragmentation And Dispersion Instrument
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