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JoVE Journal Neuroscience
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Generation of Human Brain Organoids for Mitochondrial Disease Modeling

Article DOI: 10.3791/62756-v • 08:09 min • June 21st, 2021
June 21st, 2021 •
1, 2, 1,4, 1, 2, 3, 3, 2, 1,5
1Department of General Pediatrics, Neonatology and Pediatric Cardiology, Duesseldorf University Hospital, Medical Faculty, Heinrich Heine University, 2Institute of Neurobiology, Heinrich Heine University, 3Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrueck Center for Molecular Medicine (MDC), 4Seaver Autism Center for Research and Treatment, Icahn School of Medicine at Mount Sinai, 5Max Delbrueck Center for Molecular Medicine (MDC)

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Summary
Automatic Translation

We describe a detailed protocol for the generation of human induced pluripotent stem cell-derived brain organoids and their use in modeling mitochondrial diseases.

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Brain Organoids Mitochondrial Disease Modeling Reproducible Assessment Mitochondrial Properties Bioreactors Embedding Procedures Implementation Upscaling Interventional Targets IPSCs Feeder-free Conditions Culture Humidified Tissue Culture Incubator Carbon Dioxide Passage Enzyme-free Detachment Medium Cell Survival Rho-associated Protein Kinase Inhibitor Dissociate Cortical Differentiation Medium CDM1 Pre-warm PBS Reagent A
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