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Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
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1Department of Tissue Engineering and Regeneration, School and Hospital of Stomatology, Cheeloo College of Medicine,Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 2Department of Orthodontics, School and Hospital of Stomatology,Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration, 3Department of Stomatology,Shengli Oilfield Central Hospital, 4Department of Pediatric Surgery,Shengli Oilfield Central Hospital, 5Ningbo Stomatology Hospital of Savaid Stomatology School,Hangzhou Medical College
Chapters
- 00:00Introduction
- 00:49Gingival Tissue Pretreatment
- 01:13Digestion of Gingival Tissue
- 02:46Cell Passage
- 03:51Representative Results
- 06:18Conclusion
Here we present a modified method for the isolation and culture of human gingival epithelial cells by adding the Rock inhibitor, Y-27632, to the traditional method. This method is easier, less time-consuming, enhances stem cell properties, and produces larger numbers of high-potential epithelial cells both for the laboratory and for clinical applications.
