Journal
/
Environment
/
Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
JoVE Journal
Environment
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Environment
Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

DOI:
10.3791/63101-v

•

10:03 min

•

December 07, 2021

•

,
1Department of Life Sciences, School of Natural Sciences,Shiv Nadar University

Chapters

  • 00:04Introduction
  • 00:52Sample Collection, Processing, and Analysis
  • 03:12Degradation of Hydrocarbons
  • 05:04Genomic DNA Isolation of the Pure Culture
  • 06:4916S rRNA Sequencing
  • 08:07Results: Isolation and Characterization of Hydrocarbon Degrading Bacteria from Aquatic Habitats
  • 09:11Conclusion

Summary

Automatic Translation

Automatic Translation

We present the process of isolating, propagating, and characterizing hydrocarbon-degrading bacteria from aquatic habitats. The protocol outlines bacterial isolation, identification by the 16S rRNA method, and testing of their hydrocarbon-degrading potential. This article would help researchers in characterizing microbial biodiversity in environmental samples, and specifically screen for microbes with bioremediation potential.

Tags

Keywords: Hydrocarbon-degrading MicroorganismsAquatic HabitatsIsolationCultivationIdentificationBacterial SpeciesWater SampleNutrient MediaDilutionColony ScreeningGram StainingGlycerol StocksHydrocarbon Metabolism

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63101?article_type=v

Helminth Collection and Identification from Wildlife
check_url/63101?article_type=v

Detection of Foodborne Bacterial Pathogens from Individual Filth Flies
check_url/63101?article_type=v

An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
check_url/63101?article_type=v

Characterization and Application of Passive Samplers for Monitoring of Pesticides in Water
check_url/63101?article_type=v

Experimental Protocol for Biodiesel Production with Isolation of Alkenones as Coproducts from Commercial Isochrysis Algal Biomass
check_url/63101?article_type=v

Alternative Method of Removing Otoliths from Sturgeon
check_url/63101?article_type=v

Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas
check_url/63101?article_type=v

Identification of Pharmaceuticals in The Aquatic Environment Using HPLC-ESI-Q-TOF-MS and Elimination of Erythromycin Through Photo-Induced Degradation
check_url/63101?article_type=v

Characterization of Aquatic Biofilms with Flow Cytometry
check_url/63101?article_type=v

Sampling, Sorting, and Characterizing Microplastics in Aquatic Environments with High Suspended Sediment Loads and Large Floating Debris

Read Article