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Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats
 

Isolation, Propagation, and Identification of Bacterial Species with Hydrocarbon Metabolizing Properties from Aquatic Habitats

Article DOI: 10.3791/63101-v 10:03 min December 7th, 2021
December 7th, 2021

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Summary

We present the process of isolating, propagating, and characterizing hydrocarbon-degrading bacteria from aquatic habitats. The protocol outlines bacterial isolation, identification by the 16S rRNA method, and testing of their hydrocarbon-degrading potential. This article would help researchers in characterizing microbial biodiversity in environmental samples, and specifically screen for microbes with bioremediation potential.

Tags

Isolation Propagation Identification Bacterial Species Hydrocarbon Metabolizing Properties Aquatic Habitats Microorganisms Ecological Habitats Laboratory Setup Substrates Ecological Niches Degradation Water Sample Collection PH Measurement Temperature Measurement Filtration Aseptic Conditions Filter Sheets Nutrient Media Plates Dilution Pipetting
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