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JoVE Journal Neuroscience
Elektrofysiologiske metoder til måling af fotopigmentniveauer i Drosophila Photoreceptorer
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Elektrofysiologiske metoder til måling af fotopigmentniveauer i Drosophila Photoreceptorer

Article DOI: 10.3791/63514-v • 08:09 min • June 2nd, 2022
June 2nd, 2022 •
1, 1, 1
1Department of Medical Neurobiology, Faculty of Medicine and the Edmond and Lily Safra Center for Brain Sciences (ELSC), Hebrew University

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Vi præsenterer en protokol til elektrofysiologisk at karakterisere bi-stabile fotopigmenter: (i) udnyttelse af ladningsforskydningerne i fotopigmentmolekylerne efter fotonabsorption og deres enorme mængde i fotoreceptorerne og (ii) udnyttelse af absorptionsspektreforskellene mellem rhodopsin og metarhodopsinfotopigmenttilstande. Disse protokoller er nyttige til at screene for mutationer, der påvirker bi-stabile fotopigmentsystemer.

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Neurovidenskab udgave 184
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