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Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
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Neuroscience
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JoVE Journal Neuroscience
Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

DOI:
10.3791/63776-v

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10:36 min

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March 23, 2022

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, , ,
1Department of Orthopaedics and Rehabilitation, Center for Orthopaedic Research and Translational Science (CORTS),The Pennsylvania State University College of Medicine, 2Department of Dermatology,The Pennsylvania State University College of Medicine

Chapters

  • 00:04Introduction
  • 01:17Epidermis and Dermis Separation Procedure
  • 03:03Keratinocyte Isolation from the Epidermis
  • 05:02Isolation of Schwann Cells (SCs) and Fibroblast from the Dermis
  • 08:18Results: Characterization of Human Foreskin-Derived Primary Keratinocytes, Schwann Cells, and Fibroblasts
  • 09:43Conclusion

Summary

Automatic Translation

Automatic Translation

The study of wound healing associated with musculoskeletal injury often requires the assessment of in vitro interactions between Schwann cells (SCs), keratinocytes, and fibroblasts. This protocol describes the isolation, culturing, and characterization of these primary cells from the human foreskin.

Tags

Keywords: Primary Schwann CellsKeratinocytesFibroblastsHuman ForeskinSkin CellsCell IsolationCell CultureCell InteractionsSkin HomeostasisSkin PathophysiologyWound HealingSkin Disease

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