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JoVE Journal Neuroscience
Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin
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Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

Article DOI: 10.3791/63776-v • 10:36 min • March 23rd, 2022
March 23rd, 2022 •
1, 1, 2, 1
1Department of Orthopaedics and Rehabilitation, Center for Orthopaedic Research and Translational Science (CORTS), The Pennsylvania State University College of Medicine, 2Department of Dermatology, The Pennsylvania State University College of Medicine

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Summary
Automatic Translation

The study of wound healing associated with musculoskeletal injury often requires the assessment of in vitro interactions between Schwann cells (SCs), keratinocytes, and fibroblasts. This protocol describes the isolation, culturing, and characterization of these primary cells from the human foreskin.

Tags

Isolation Culture Primary Schwann Cells Keratinocytes Fibroblasts Human Foreskin In Vitro Experiments Individual Cell Cultures Genetic Variation Skin Homeostasis Pathophysiology Co-culture Experiments Skin Physiology Wound Healing Skin Diseased States Neonatal Foreskin DMEM Basal Medium Cell Isolation DPBS Antibiotics Antimycotic
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