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Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection
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Immunology and Infection
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JoVE Journal Immunology and Infection
Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

Experimental Viral Infection in Adult Mosquitoes by Oral Feeding and Microinjection

DOI:
10.3791/63830-v

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08:02 min

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July 28, 2022

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, , , , , ,
1Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology,Chinese Academy of Sciences, 2University of Chinese Academy of Sciences

Chapters

  • 00:04Introduction
  • 00:58Performing Oral Infection
  • 02:32Intrathoracic Inoculation
  • 04:02Viral Injection Under a Dissecting Microscope
  • 04:53Processing of the Infected Female Mosquitoes
  • 06:42Results: Determination of Viral Infection in Salivary Glands of Aedes aegypti
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

This methodology, which included oral feeding and intrathoracic injection infection, could effectively assess the influence of midgut and/or salivary gland barriers on arbovirus infection.

Tags

Viral InfectionMosquitoesOral FeedingMicroinjectionAedes AegyptiArbovirusVector CompetenceDengue VirusZika VirusArtificial Feeding SystemAnesthesiaIncubationIntrathoracic InoculationVirus DilutionMicroinjection Needles

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