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Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk
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Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk

Viral Transgene Expression in Rodent Hearts and the Assessment of Cardiac Arrhythmia Risk

DOI:
10.3791/64073-v

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05:15 min

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July 27, 2022

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, , , , , , , ,
1University of Ottawa Heart Institute, 2Department of Cellular and Molecular Medicine,University of Ottawa, 3Department of Biochemistry, Microbiology and Immunology,University of Ottawa

Chapters

  • 00:05Introduction
  • 00:51Viral Transgene Expression in Rodent Ventricular Tissues
  • 01:37Assessment of Cardiac Arrhythmia Susceptibility
  • 03:47Results: Assessment of Cardiac Susceptibility to Arrhythmias
  • 04:35Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol describes methods for transgene expression in rat and mouse hearts by direct intramyocardial injection of the virus under echocardiography guidance. Methods for the assessment of the susceptibility of hearts to ventricular arrhythmias by the programmed electrical stimulation of isolated, Langendorff-perfused hearts are also explained here.

Tags

Keywords: Viral Transgene ExpressionVentricular MyocardiumCardiac ArrhythmiaLangendorff PerfusionECGIsoproterenolProgrammed Electrical StimulationVentricular Tachyarrhythmia

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