JoVE Journal
Biochemistry
Biochemistry
A subscription to JoVE is required to view this content.
使用Leginon进行载物台倾斜的单颗粒冷冻电镜数据收集
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
Click here for the English version
Article
DOI:
10.3791/64136-v
•
04:52 min
•
July 1st, 2022
使用Leginon进行载物台倾斜的单颗粒冷冻电镜数据收集
July 1st, 2022
•
Sriram Aiyer1, Timothy S. Strutzenberg1, Marianne E. Bowman2, Joseph P. Noel2,3, Dmitry Lyumkis1,4,5
1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry, University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology, University of California San Diego, 5Department of Integrative Structural and Computational Biology, The Scripps Research Institute
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
本协议描述了冷冻电镜实验中倾斜单颗粒数据收集的通用且易于实施的方案。对于由于粘附空气-水界面而遭受优先方向偏差的样品,该程序对于获得高质量的EM图特别有用。