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Isolation of Regenerating Hepatocytes after Partial Hepatectomy in Mice
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Regenerating Hepatocytes after Partial Hepatectomy in Mice
DOI:

10:04 min

December 02, 2022

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Chapters

  • 00:04Introduction
  • 00:43Preparation of Perfusion Equipment and Animal Surgery
  • 02:51Cannulation and Perfusion
  • 05:03Digestion
  • 06:21Preparation of the Liver
  • 06:51Hepatocyte Extraction and Isolation
  • 08:11Results: A Reliable and Straightforward Method for Isolating High Yield Normal and Steatotic Murine Hepatocytes
  • 09:37Conclusion

Summary

Automatic Translation

Lipid-laden hepatocytes are inherent to liver regeneration but are usually lost upon density-gradient centrifugation. Here, we present an optimized cell isolation protocol that retains steatotic hepatocytes, yielding representative populations of regenerating hepatocytes after partial hepatectomy in mice.

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