3,576 Views
•
11:44 min
•
March 24, 2023
DOI:
Summary
Automatically generated
The electroporation of primate cerebral organoids provides a precise and efficient approach to introduce transient genetic modification(s) into different progenitor types and neurons in a model system close to primate (patho)physiological neocortex development. This allows the study of neurodevelopmental and evolutionary processes and can also be applied for disease modeling.
Related Videos
08:14
Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
Related Videos
7538 Views
08:48
Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects
Related Videos
9237 Views
10:02
Ex Vivo Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors
Related Videos
9277 Views
08:03
Simultaneous Assessment of Cardiomyocyte DNA Synthesis and Ploidy: A Method to Assist Quantification of Cardiomyocyte Regeneration and Turnover
Related Videos
10382 Views
08:51
Microinjection for Transgenesis and Genome Editing in Threespine Sticklebacks
Related Videos
13952 Views
13:12
Efficient Gene Delivery into Multiple CNS Territories Using In Utero Electroporation
Related Videos
19834 Views
07:13
Electroporation of Sliced Human Cortical Organoids for Studies of Gene Function
Related Videos
1081 Views
00:06
Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains
Related Videos
5933 Views
09:42
Injections of AAV Vectors for Optogenetics in Anesthetized and Awake Behaving Non-Human Primate Brain
Related Videos
3610 Views
08:58
A Human Cerebral Organoid Model of Neural Cell Transplantation
Related Videos
1161 Views
Read Article
Cite this Article
Tynianskaia, L., Eşiyok, N., Huttner, W. B., Heide, M. Targeted Microinjection and Electroporation of Primate Cerebral Organoids for Genetic Modification. J. Vis. Exp. (193), e65176, doi:10.3791/65176 (2023).
Copy