A Procedure for Mouse Dorsal Root Ganglion Cryosectioning

Liangliang He; Wenxing Zhao; Lingyi Zhang; Maalveka Ilango; Na Zhao; Liqiang Yang; Zhonghui Guan

doi:10.3791/65232

Journal

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Neuroscience

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A Procedure for Mouse Dorsal Root Ganglion Cryosectioning

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

A Procedure for Mouse Dorsal Root Ganglion Cryosectioning

5,713 Views

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04:20 min

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June 09, 2023

DOI:

10.3791/65232-v

DOI:

10.3791/65232-v

•

04:20 min

June 09, 2023

•

78 Views

Liangliang He*^1,2, Wenxing Zhao*¹, Lingyi Zhang^2,3, Maalveka Ilango^2,4, Na Zhao², Liqiang Yang¹, Zhonghui Guan²

¹Department of Pain Management, Xuanwu Hospital,Capital Medical University, ²Department of Anesthesia and Perioperative Care,University of California San Francisco, ³Department of Anesthesiology,Sun Yat-Sen University, ⁴University of Washington

Transcript

It remains a challenge to consistently obtain high-quality, intact, and flat mouse dorsal root ganglion cryostat sections. The research aims to answer how to acquire ideal mouse dorsal root ganglion cryostat section. This protocol provides an easy step-by-step technique for cryostat section of the mouse DRG to high quality, intact, and flat mouse DRG cryostat sections.

This presented article explains how to remove surrounding liquid from the mouse DRG sample tissue, placing the DRG sections on the slides facing the same orientation, and flatten the DRG sections without curving up. One major challenge is that the OCT sections of mouse DRG tend to curve into a roll, making it difficult to place onto the glass slide. It is also challenging to acquire sufficient sections from one sample due to the small size of the mouse DRG.