Fabrication and Characterization of Colorectal Cancer Organoids from SW1222 Cell Line in Ultrashort Self-Assembling Peptide Matrix

Our research aims to evaluate ultrashort self-assembling peptides as matrices for colorectal cancer organoid cultures. We address questions about organoid morphology for viability, proliferation, and the adhesion and the impact of biofunctionalization. The goal is to optimize peptide matrix compositions for effective organoid growth and to contribute to regenerative medicine.

Cutting-edge technologies in our field involve 3D bioprinting for precise organic fabrication, microfluidic platforms for dynamic culture environments, and advanced imaging tools like atomic force microscopy and multiphoton microscopy. CRISPR-Cas9 gene editing refines genetic modification while single-cell RNA sequencing provides in-depth molecular insights. Current experimental challenges include enhancing organoid reproducibility, refining vascularization for larger constructs, and mimicking complex tissue architecture.