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High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor
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1Department of Coloproctological Surgery,Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis,Juntendo University Faculty of Medicine, 3Atopy Research Center,Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery,Tokyo Dental College, 5Department of Gastroenterology,Juntendo University Faculty of Medicine
Chapters
- 00:04Title
- 00:57Colorectal Cancer Cell (CRC) Organoid Artificial Extracellular Matrix Culture Installation
- 02:42CRC Organic Cell-labeling with GFP Lentiviral Particles
- 05:28GFP-labeled CRC Organoid Metastasis Generation
- 07:55Results: Representative High-resolution GFP Visualization in Cultured GFP-labeled CRC Organoids, Primary Tumors, and Micrometastases
- 08:38Conclusion
To allow highly sensitive detection of the disseminating human colorectal cancer (CRC) cells colonizing tissues, we herein show a protocol for efficient transduction of green fluorescent protein (GFP) lentiviral particles into PDX-derived CRC organoid cells prior to their injection into recipient mice, with stereo-fluorescence microscopic observation.
