Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content.
You will only be able to see the first 20 seconds.

JoVE Journal Cancer Research
High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor
 

High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor

Article doi: 10.3791/57374
June 14th, 2018 • Usage Statistics
1,2, 1,2, 2,4, 5, 1, 1, 1, 3, 2, 1, 2
1Department of Coloproctological Surgery, Juntendo University Faculty of Medicine, 2Department of Molecular Pathogenesis, Juntendo University Faculty of Medicine, 3Atopy Research Center, Juntendo University Faculty of Medicine, 4Department of Oral Pathobiological Science and Surgery, Tokyo Dental College, 5Department of Gastroenterology, Juntendo University Faculty of Medicine

Summary
Automatic Translation
June 14th, 2018

To allow highly sensitive detection of the disseminating human colorectal cancer (CRC) cells colonizing tissues, we herein show a protocol for efficient transduction of green fluorescent protein (GFP) lentiviral particles into PDX-derived CRC organoid cells prior to their injection into recipient mice, with stereo-fluorescence microscopic observation.

Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter