High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor

Yu Okazawa; Kosuke Mizukoshi; Yu Koyama; Shoki Okubo; Hiromitsu Komiyama; Yutaka Kojima; Michitoshi Goto; Sonoko Habu; Okio Hino; Kazuhiro Sakamoto; Akira Orimo

doi:10.3791/57374

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High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor

10,029 Views

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09:16 min

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June 14, 2018

DOI:

10.3791/57374-v

DOI:

10.3791/57374-v

•

09:16 min

June 14, 2018

•

9992 Views

Yu Okazawa^1,2, Kosuke Mizukoshi^1,2, Yu Koyama^2,4, Shoki Okubo⁵, Hiromitsu Komiyama¹, Yutaka Kojima¹, Michitoshi Goto¹, Sonoko Habu³, Okio Hino², Kazuhiro Sakamoto¹, Akira Orimo²

¹Department of Coloproctological Surgery,Juntendo University Faculty of Medicine ²Department of Molecular Pathogenesis,Juntendo University Faculty of Medicine ³Atopy Research Center,Juntendo University Faculty of Medicine ⁴Department of Oral Pathobiological Science and Surgery,Tokyo Dental College ⁵Department of Gastroenterology,Juntendo University Faculty of Medicine

Summary

To allow highly sensitive detection of the disseminating human colorectal cancer (CRC) cells colonizing tissues, we herein show a protocol for efficient transduction of green fluorescent protein (GFP) lentiviral particles into PDX-derived CRC organoid cells prior to their injection into recipient mice, with stereo-fluorescence microscopic observation.