October 11th, 2024
Here, we present a protocol for evaluating the surface characteristics of endodontic retreatment files after repeated use in retreatment procedures, utilizing scanning electron microscopy to identify and analyze potential surface defects.
The aim of our search is to investigate the impact of repeated use of a remover file on it surface characteristic with a particular focus on the number of users. The objective is to determine the number of times the product can be safely used without compromising its surface integrity. Our significant findings indicate that the remover treatment file can be safely used for up to three treatment cycles without compromising its performance or patient safety.
In the future, our laboratory plans to focus on conducting similar studies in clinical settings are feasible on human teeth. This will help enhance reliability of our results and further validate our findings in real-world applications. To begin procure 80 acrylic blocks with an internal diameter of 1.5 millimeters, a radius of curvature of 5 millimeters and a 55 degree curvature.
After cleaning and shaping the blocks, operate the files at 600 RPM and 2.5 Newton-Centimeter torque according to the manufacturer's instructions. Use the files with a back and forth motion without applying apical pressure until they are 3 millimeters short of the working length. Then remove the file from the canal when resistance is felt and irrigate the canal with 5.25%sodium hypochlorite solution.
Once the scanning electron microscopy is complete, have two calibrated examiners analyze all images on a computer screen and record the presence and type of deformation such as tip deformation, micro cracks, fracture, unwinding, surface pitting, and blade disruption. Deformation was observed in 75%of the files after a single use and in 100%of the files after three and six uses with no statistically significant difference between the groups. Micro cracks were not observed after a single use, but were present in 25%of the files after three uses and an 87.5%of the files after six uses showing a statistically significant increase.
Surface pitting was observed in 25%of the files after a single use, 87.5%after three uses, and 100%after six uses with statistically significant differences between the groups. No significant differences were observed between the groups for unwinding, tip deformation and blade disruption. No fractures were observed in any of the groups.
This study investigates the surface characteristics of endodontic retreatment files after repeated use, focusing on their integrity and safety. Using scanning electron microscopy, the research aims to identify potential surface defects that may arise from multiple treatment cycles.